2024
Atefeh Alipour Ahmad Hasanzadeh, Sajedeh Ghasemi
In: Journal of Science: Advanced Materials and Devices, vol. 9, iss. 4, 2024.
@article{nokey,
title = {Proanthocyanidin-Imbued Cellulosic 3-Dimentional Intrinsic Aligned Nanostructures: A Novel Approach for Dental and Bone Regeneration using Dental Pulp Derived Stem Cells },
author = {Ahmad Hasanzadeh, Atefeh Alipour, Sajedeh Ghasemi, Saadi Hosseini, Naser Farrokhi, Peng-Yuan Wang, Ali Zarrabi, Javad Mohammadi, Hosein Shahsavarani},
doi = {https://doi.org/10.1016/j.jsamd.2024.100820},
year = {2024},
date = {2024-12-01},
journal = { Journal of Science: Advanced Materials and Devices},
volume = {9},
issue = {4},
abstract = {Developing effective scaffolds to address significant bone and dental defects is crucial in regenerative osteal and dental medicine. Traditional methods utilizing synthetic micropatterned scaffolds have effectively stimulated osteogenic and odontogenic differentiation of stem cells through parallel, 3D topographic, hexagonal, and elongated architectural features. However, these approaches face significant cost, scalability, and biocompatibility challenges. Recent advancements have highlighted the potential of decellularized plant scaffolds, such as those derived from Beaucarnea recurvata leaves (BLDS), which offer intrinsic microstructural advantages with solving reproducibility, scalability, incurred cost, and biocompatibility challenges. This study explores the enhancement of BLDS using grape seed proanthocyanidin extract (GSPE), a natural polyphenol known for its beneficial effects on bone and dental stem cell differentiation. By functionalizing BLDS with GSPE, we investigated its impact on osteogenic and odontogenic differentiation of human dental pulp-derived mesenchymal stem cells (DPDMSCs). The modified scaffolds exhibited improved physicochemical properties, including enhanced cell proliferation, protein absorption, scaffold interactions, and upregulated osteogenic and dental marker gene expression. SEM imaging revealed significant cellular growth and morphological changes indicative of successful differentiation. Furthermore, BLDS-GSPE demonstrated increased ALP activity and mineral deposition, suggesting its potential as a cost-effective, reproducible and biocompatible alternative for bone and dental repair compared to conventional synthetic biomaterials. },
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Developing effective scaffolds to address significant bone and dental defects is crucial in regenerative osteal and dental medicine. Traditional methods utilizing synthetic micropatterned scaffolds have effectively stimulated osteogenic and odontogenic differentiation of stem cells through parallel, 3D topographic, hexagonal, and elongated architectural features. However, these approaches face significant cost, scalability, and biocompatibility challenges. Recent advancements have highlighted the potential of decellularized plant scaffolds, such as those derived from Beaucarnea recurvata leaves (BLDS), which offer intrinsic microstructural advantages with solving reproducibility, scalability, incurred cost, and biocompatibility challenges. This study explores the enhancement of BLDS using grape seed proanthocyanidin extract (GSPE), a natural polyphenol known for its beneficial effects on bone and dental stem cell differentiation. By functionalizing BLDS with GSPE, we investigated its impact on osteogenic and odontogenic differentiation of human dental pulp-derived mesenchymal stem cells (DPDMSCs). The modified scaffolds exhibited improved physicochemical properties, including enhanced cell proliferation, protein absorption, scaffold interactions, and upregulated osteogenic and dental marker gene expression. SEM imaging revealed significant cellular growth and morphological changes indicative of successful differentiation. Furthermore, BLDS-GSPE demonstrated increased ALP activity and mineral deposition, suggesting its potential as a cost-effective, reproducible and biocompatible alternative for bone and dental repair compared to conventional synthetic biomaterials.
0000
Ahmad Hasanzadeh Sajedeh Ghasemi, Hosein Shahsavarani
Investigating the Effect of the Structure of Plant-Based Scaffolds Modified with Epigallocatechin Gallate on the Fate of Dental Mesenchymal Stem Cells Unpublished Forthcoming
Forthcoming, (In progress).
@unpublished{nokey,
title = {Investigating the Effect of the Structure of Plant-Based Scaffolds Modified with Epigallocatechin Gallate on the Fate of Dental Mesenchymal Stem Cells},
author = {Sajedeh Ghasemi, Ahmad Hasanzadeh, Hosein Shahsavarani, Atefeh Alipour},
abstract = {Various methods have been proposed for fabricating three-dimensional scaffolds to provide suitable conditions for the growth of stem cells. In addition to the scaffold's structure, another effective factor in cell growth is growth factors. These materials have created challenges for researchers due to their high production cost and potential long-term negative side effects.
In this study, a decellularized Haworthia plant scaffold coated with the compound epigallocatechin gallate (EGCG) was used for culturing dental mesenchymal stem cells. This approach not only provides a suitable three-dimensional structure but also avoids the problems associated with synthetic scaffolds and is more economically viable. Furthermore, EGCG, a naturally sourced compound with osteoinductive properties, was used as an alternative to growth factors to guide the growth and differentiation of dental mesenchymal stem cells.
The surface properties of the scaffolds were studied before and after coating by measuring hydrophilicity, examining surface microstructure, porosity percentage, and mechanical properties. In the next stage, the osteogenic differentiation ability of dental mesenchymal stem cells cultured on the coated scaffold, a culture dish containing osteogenic factors (positive control sample), and a culture dish containing cells (control sample) was evaluated using Alizarin Red staining, measurement of alkaline phosphatase activity, and analysis of osteogenic gene expression.
The results showed that the use of EGCG, in combination with the suitable three-dimensional structure and mechanical properties of the scaffold, was effective in the adhesion, growth, and differentiation of the stem cells. EGCG's ability to induce osteogenic differentiation, in addition to optimizing surface hydrophilicity and providing a suitable surface-to-volume ratio, led to an increase in the efficiency of dental mesenchymal stem cell differentiation toward an osteogenic fate.
Based on the findings, it can be concluded that the decellularized Haworthia plant scaffold coated with EGCG can be used as a suitable structure for the growth and differentiation of dental cells in dental tissue engineering due to its accessibility, low production cost, and high efficiency.},
note = {In progress},
keywords = {},
pubstate = {forthcoming},
tppubtype = {unpublished}
}
Various methods have been proposed for fabricating three-dimensional scaffolds to provide suitable conditions for the growth of stem cells. In addition to the scaffold's structure, another effective factor in cell growth is growth factors. These materials have created challenges for researchers due to their high production cost and potential long-term negative side effects.
In this study, a decellularized Haworthia plant scaffold coated with the compound epigallocatechin gallate (EGCG) was used for culturing dental mesenchymal stem cells. This approach not only provides a suitable three-dimensional structure but also avoids the problems associated with synthetic scaffolds and is more economically viable. Furthermore, EGCG, a naturally sourced compound with osteoinductive properties, was used as an alternative to growth factors to guide the growth and differentiation of dental mesenchymal stem cells.
The surface properties of the scaffolds were studied before and after coating by measuring hydrophilicity, examining surface microstructure, porosity percentage, and mechanical properties. In the next stage, the osteogenic differentiation ability of dental mesenchymal stem cells cultured on the coated scaffold, a culture dish containing osteogenic factors (positive control sample), and a culture dish containing cells (control sample) was evaluated using Alizarin Red staining, measurement of alkaline phosphatase activity, and analysis of osteogenic gene expression.
The results showed that the use of EGCG, in combination with the suitable three-dimensional structure and mechanical properties of the scaffold, was effective in the adhesion, growth, and differentiation of the stem cells. EGCG's ability to induce osteogenic differentiation, in addition to optimizing surface hydrophilicity and providing a suitable surface-to-volume ratio, led to an increase in the efficiency of dental mesenchymal stem cell differentiation toward an osteogenic fate.
Based on the findings, it can be concluded that the decellularized Haworthia plant scaffold coated with EGCG can be used as a suitable structure for the growth and differentiation of dental cells in dental tissue engineering due to its accessibility, low production cost, and high efficiency.
In this study, a decellularized Haworthia plant scaffold coated with the compound epigallocatechin gallate (EGCG) was used for culturing dental mesenchymal stem cells. This approach not only provides a suitable three-dimensional structure but also avoids the problems associated with synthetic scaffolds and is more economically viable. Furthermore, EGCG, a naturally sourced compound with osteoinductive properties, was used as an alternative to growth factors to guide the growth and differentiation of dental mesenchymal stem cells.
The surface properties of the scaffolds were studied before and after coating by measuring hydrophilicity, examining surface microstructure, porosity percentage, and mechanical properties. In the next stage, the osteogenic differentiation ability of dental mesenchymal stem cells cultured on the coated scaffold, a culture dish containing osteogenic factors (positive control sample), and a culture dish containing cells (control sample) was evaluated using Alizarin Red staining, measurement of alkaline phosphatase activity, and analysis of osteogenic gene expression.
The results showed that the use of EGCG, in combination with the suitable three-dimensional structure and mechanical properties of the scaffold, was effective in the adhesion, growth, and differentiation of the stem cells. EGCG's ability to induce osteogenic differentiation, in addition to optimizing surface hydrophilicity and providing a suitable surface-to-volume ratio, led to an increase in the efficiency of dental mesenchymal stem cell differentiation toward an osteogenic fate.
Based on the findings, it can be concluded that the decellularized Haworthia plant scaffold coated with EGCG can be used as a suitable structure for the growth and differentiation of dental cells in dental tissue engineering due to its accessibility, low production cost, and high efficiency.